Efecto in vitro de 2 compuestos bioactivos, el ácido gálico y el galato de metilo, sobre la urolitiasis / In vitro effects of 2 bioactive compounds, gallic acid and methyl gallate, on urolithiasis

Introducción y objetivos: El objetivo de este estudio fue evaluar el papel de 2 compuestos fenólicos naturales ampliamente distribuidos, el ácido gálico (AG) y el galato de metilo (GM), en un modelo in vitro de urolitiasis, utilizando la metodología de formación de cristales de oxalato de calcio, el tipo más común de cálculos urinarios o renales.Material y métodosLos compuestos AG y GM fueron sometidos a actividades de «anticristalización» en diferentes concentraciones (0,003-0,03mg/mL), y la cantidad y morfología de los cristales se determinó por microscopia después de 60min.ResultadosEl AG inhibió alrededor del 44-57% de la formación total de cristales de cristales de oxalato de calcio, mientras que el GM inhibió alrededor del 48,35%, en comparación con las muestras expuestas al vehículo (agua destilada; grupo de control negativo). La exposición a AG y GM inhibió la formación de cálculos de tipo monohidrato, considerada la categoría de cristales más común y dañina. Los compuestos también disminuyeron la absorbancia, lo que a su vez está relacionado con una reducción de la agregación y precipitación de cristales de oxalato de calcio.ConclusionesEn conjunto, este estudio muestra, por primera vez, que el AG y el GM son compuestos prometedores con propiedades antiurolitiásicas, brindando nuevas perspectivas para futuros estudios in vivo del potencial de estos compuestos en el tratamiento y/o prevención de los cálculos urinarios o renales. (AU)

Introduction and objectives: This study aimed to evaluate the role of 2 widely distributed natural phenolic compounds, gallic acid (GA) and methyl gallate (MG), in an in vitro model of urolithiasis, by using the methodology of calcium oxalate crystals formation, which is the most common type of urinary or kidney stones.Material and methodsThe compounds GA and MG were subjected to anti-crystallization activities in different concentrations (0.003-0.03mg/mL), and the quantity and morphology of crystals were determined by microscopy after 60min.ResultsGA inhibited about 44-57% of the total calcium oxalate crystals formation, while MG inhibited about 48.35%, when compared to vehicle-exposed samples (distilled water; negative control group). GA and MG exposure inhibited monohydrate type calculi formation, which is considered the most common and harmful crystal category. The compounds also decreased absorbance, which in turn is related to reduced calcium oxalate crystals aggregation and precipitation.ConclusionsAltogether, this study shows, for the first time, that GA and MG are promising compounds with antiurolithiatic properties, opening new perspectives for future in vivo evaluations of the potential of these compounds in the treatment and/or prevention of urinary or kidney stones. (AU)

In vitro effects of two bioactive compounds, gallic acid and methyl gallate, on urolithiasis.

INTRODUCTION AND OBJECTIVES: This study aimed to evaluate the role of two widely distributed natural phenolic compounds, gallic acid (GA) and methyl gallate (MG), in an in vitro model of urolithiasis, by using the methodology of calcium oxalate (CaOx) crystals formation, which is the most common type of urinary or kidney stones. MATERIAL AND METHODS: The compounds GA and MG were subjected to anti-crystallization activities in different concentrations (0.003-0.03 mg/mL), and the quantity and morphology of crystals were determined by microscopy after 60 min. RESULTS: GA inhibited about 44-57% of the total CaOx crystals formation, while MG inhibited about 48.35%, when compared to vehicle-exposed samples (distilled water; negative control group). GA and MG exposure inhibited monohydrate type calculi formation, which is considered the most common and harmful crystal category. The compounds also decreased absorbance, which in turn is related to reduced CaOx aggregation and precipitation. CONCLUSIONS: Altogether, this study shows, for the first time, that GA and MG are promising compounds with antiurolithiatic properties, opening new perspectives for future in vivo evaluations of the potential of these compounds in the treatment and/or prevention of urinary or kidney stones.

In vitro effects of 2 bioactive compounds, gallic acid and methyl gallate, on urolithiasis. / Efecto in vitro de 2 compuestos bioactivos, el ácido gálico y el galato de metilo, sobre la urolitiasis.

INTRODUCTION AND OBJECTIVES: This study aimed to evaluate the role of 2 widely distributed natural phenolic compounds, gallic acid (GA) and methyl gallate (MG), in an in vitro model of urolithiasis, by using the methodology of calcium oxalate crystals formation, which is the most common type of urinary or kidney stones. MATERIAL AND METHODS: The compounds GA and MG were subjected to anti-crystallization activities in different concentrations (0.003-0.03mg/mL), and the quantity and morphology of crystals were determined by microscopy after 60min. RESULTS: GA inhibited about 44-57% of the total calcium oxalate crystals formation, while MG inhibited about 48.35%, when compared to vehicle-exposed samples (distilled water; negative control group). GA and MG exposure inhibited monohydrate type calculi formation, which is considered the most common and harmful crystal category. The compounds also decreased absorbance, which in turn is related to reduced calcium oxalate crystals aggregation and precipitation. CONCLUSIONS: Altogether, this study shows, for the first time, that GA and MG are promising compounds with antiurolithiatic properties, opening new perspectives for future in vivo evaluations of the potential of these compounds in the treatment and/or prevention of urinary or kidney stones.